The role of PTTH in the initiation of adult development.

<p>(A) The effect of brain extract (Br-ex) injection. The Br-CC-CA or brain alone (Br) was removed from post-diapause pupae 12 h after warming and 12 h later, brain extract (2 units of PTTH equivalents) was injected. As a negative control, saline was injected into the brain-deficient pupae. (B) The effect of PTTH removal from the brain extract. The brains were removed from post-diapause pupae 12 h after warming, and the debrained pupae were injected with the brain extract pre-treated with an anti-<i>Mab</i>PTTH or control antibody. As a negative and a positive controls, saline and the same dose (3 units of PTTH equivalents) of the brain extract were injected, respectively (n = 6). The values shown are the means (± SEM) of three independent determinations. (C) The Effect of the implantation of PTTH-containing gel into the Br-CC-CA-deficient pupae. The Br-CC-CA was removed from post-diapause pupae immediately after warming, and the PTTH-containing gel (+PTTH) was implanted into the Br-CC-CA-deficient pupae (n = 8). Control pupae received the gel without PTTH (-PTTH).