The role of the Br-CC-CA in post-diapause development.

<p>(A) The effects of brain removal and implantation on adult development of post-diapause pupae. Diapausing pupae were chilled for 8 weeks and the Br-CC-CA was removed immediately after warming. A Br-CC-CA collected from another previously chilled pupa was implanted into a Br-CC-CA-deficient pupa. As a positive control, pupae were only injured (sham-operated). For comparison, a Br-CC-CA collected from an unchilled pupa 5 weeks after pupation (*) was implanted into a Br-CC-CA-deficient pupa. Percentage values were determined with 6–12 pupae per operation. The values shown are the means (±SEM) of three independent determinations. (B) Time-dependent effects of brain removal on the development of post-diapause pupae. The Br-CC-CA was removed from post-diapause pupae at various times after warming (n = 12). (C) The effect of CC-CA removal (allatectomy) on adult development of post-diapause pupae. The CC-CA was removed immediately after warming. Percentage values were determined with 6–12 pupae per operation. The values shown are the means (±SEM) of three independent determinations.