Effects of AjAPN1 RNAi on <i>A. janata</i> development.

<p>Third instar larvae were intrahemocoelically injected with target and control gene siRNA duplexes at dose of 5 µg/100 mg body weight. The larval growth rate was monitored by recording the weight of each larva every 24 h for 5 days. Larval mortality was calculated 2 day post-injection. Percentage of successful pupation and pupal weights were compared between the control and experimental groups. Values represented are mean±standard deviation of three independent experiments (n = 3). Significance between groups was tested by One-Way ANOVA followed by SNK test using SigmaPlot 11.0 software. *indicate statistical significance (P<0.05). (A) Larval mortality. Arrow indicates non-feeding inactive/death larvae. (B) Inhibition of larval growth. (C) Percentage of successful normal pupation. (D) Pupal weight. (E) Development of lethal larval-pupal intermediates. (F) Emergence of viable defective adults.