Treatment with dsRNA for <i>TcVer</i>, <i>TcCPAP1-C, TcCPAP1-H</i> or <i>TcCPAP1-J</i> affects pupal and adult development.

<p>The phenotypes produced by injection of dsRNAs for <i>CPAP1</i> genes at the penultimate and/or last instar larval stages are shown along with the phenotype of the <i>Vermilion (Ver)</i> dsRNA-injected control. Gene-specific dsRNAs for each of the three indicated <i>CPAP1</i> genes were injected into penultimate instar larvae (200 ng per insect; n = 40). The terminal phenotypes of insects after RNAi for <i>TcCPAP1-C, TcCPAP1-H</i> and <i>TcCPAP1-J</i> are shown. A red slash across the figure indicates that these are phenotypes of moribund animals. They all molted normally during earlier stages but exhibited developmental arrest at the pupal to adult molt and 100% lethality. White arrows indicate incomplete cuticle slippage. dsRNA <i>TcVer</i>-treated controls developed into adults with no mortality and had no abnormalities except for the expected loss of eye pigmentation.