Effect of CLA supplementation on steroidogenic genes and proteins <i>in</i><i>vitro</i>.
- Rosario Barone (479913)
- Filippo Macaluso (479914)
- Patrizia Catanese (479915)
- Antonella Marino Gammazza (451303)
- Luigi Rizzuto (479916)
- Paola Marozzi (479917)
- Giuseppe Lo Giudice (342086)
- Tomaso Stampone (479918)
- Francesco Cappello (55681)
- Giuseppe Morici (479919)
- Giovanni Zummo (314374)
- Felicia Farina (314371)
- Valentina Di Felice (370801)
Publication date
November 2013
DOIAbstract
<p>A. Real-time PCR analysis of genes encoding steroidogenic enzymes from <i>in </i><i>vitro</i> R2C cell extract. The graph shows the normalisation with the reference genes, according to the Livak Method (2-∆∆CT). B. Representative western blots showing CYP17A1 (55 kDa) and ß-actin (42 kDa) expression following electrophoretic separation of protein extracts obtained from R2C cells treated with different concentrations of CLA (w/o, 0.5, 1.5 and 7.5 µM CLA). C. Relative amounts of CYP17A1. † Significant difference compared to w/o CLA (P<0.01). </p
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