Effects of CYP-treatment on steroidogenesis-related genes and media T levels in mLTC-1 cells.

<p>(A) The mRNA levels of mLTC-1 steroidogenesis-related genes. (B) The protein levels of mLTC-1 steroidogenesis-related genes. (C) Quantitative analysis of scanning densitometry of protein levels of mLTC-1 steroidogenesis-related genes from (B). (D) Media T levels of mLTC-1 cells. The mRNA and protein levels of Star, 3β-HSD, Cyp17a1, and 17β-HSD3 were downregulated significantly compared with the control group. The mRNA and protein levels of Cyp19a1 were upregulated after CYP treatment. The media T levels were downregulated. LH was used as a positive control. The data represent the mean ± SEM. *<i>P</i><0.05. C, CYP.