<i>t10c12</i> CLA reduces HER2 protein in SK-Br3 cells.

<p>(A) Representative western blot of total HER2 protein in response to 24 hr treatment with <i>t10c12</i> CLA. Cells were plated in 6-well plates, 3 wells per treatment. Cells from 3 wells were pulled and total protein was extracted as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005342#s2" target="_blank">Materials and Methods</a>. 25 µg of whole cell lysate were loaded into 8% gels. Densitometry of bands was performed using Scion Image software Alpha 4.0.3.2. Expression of HER2 was normalized to beta actin and compared to vehicle treatment. Values represent the mean +/− std error relative to vehicle from 5 independent experiments. (B) Immunofluoresnce of HER2 protein in SK-Br3 cells following 24 hr treatment with 40 µM (middle panel), 80 µM (right panel) or vehicle (left panel). Cells were treated and immunofluorescence was performed as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005342#s2" target="_blank">Materials and Methods</a>. Images were obtained using Delta Vision deconvolution microscope with SoftwoRX 3.5.0 software at 40× and optimized using Adobe PhotoShop CS2 version 9.0.2.