Immunofluorescence and quantitation of <i>SIM1</i> neurons in PVN and SON of iDTR and Sim1Cre mice.

<p>(A) PVN of iDTR mice shows robust expression of <i>SIM1</i>, (B) <i>SIM1</i> staining was dramatically decreased in PVN of Sim1creiDTR mice. Robust expression of <i>SIM1</i> was observed in SON of both iDTR (C) and Sim1creiDTR mice (D). (E) Quantitation of <i>SIM1</i> positive neurons in PVN and SON reveals similar numbers of <i>SIM1</i> positive neurons in SON of both iDTR and Sim1Cre mice but a significant decrease in SIM1 neurons of PVN of Sim1Cre mice relative to iDTR mice (n=3 for each group, * p<0.05). Scale bar: 40 µm for A, B; and 20 µm for C, D.