Reduced levels of ER and UPR-related proteins in 14-3-3ζtg mice.

<p>(A) Representative KDEL (green) immunostaining for wt and 14-3-3ζtg mice hippocampus. Neurons are identified by NeuN counterstaining (red). Merged panels confirm staining is neuronal. Note, lower KDEL immunoreactivity in 14-3-3ζtg mice. Scale bar (top), 700 µm; (bottom), 150 µm. (B) Representative western blots (<i>n</i> = 1 per lane) showing KDEL-containing proteins in microdissected subfields of wt and 14-3-3ζtg mice. (C, D) Basal Grp78 and Grp94 protein levels in wt and 14-3-3ζtg mice (<i>n</i> = 3 per group). (E) Real-time PCR measurement of <i>Grp78</i> levels in wt and 14-3-3ζtg mice in each subfield (<i>n</i> = 3 per group). (F) Western blots (<i>n</i> = 1 per lane) showing levels of various ER-related proteins in the CA3 subfield of 14-3-3ζtg mice compared to wt mice. (G) Graphs showing lower basal levels of p-eIF2α, ATF6 and ATF4 in select hippocampal subfields (<i>n</i> = 3 per group). (H) Gel showing levels of the spiced form of <i>Xbp1</i> from hippocampal lysates of; (lane 2) WT mice subject to seizures levels, (lane 3) Wt mice, (lane 4) 14-3-3ζtg mice. Lane 1 is a ladder. *<i>p</i><0.05 compared to wt.