Disrupting actin and microtubule organization does not affect ECM organization.

<p>Confocal z-stack image reconstructions of ECFCs seeded on fibrin microfibers for 24 hrs followed by treatment with (<b>A</b>) cytochalasin D or (<b>B</b>) nocodazole for 24 hrs and 48 hrs in culture. (<b>C</b>) Low (left) and high (right) magnification of ECFCs seeded on fibrin microfibers for 72 hrs without drug treatment, serving as control. F-actin (phalloidin) is shown in green, microtubules (α-tubulin) in red, ECM proteins (collagen IV or fibronectin) in red or magenta, and nuclei in blue. Yellow arrows indicate the direction of nanotopography. Scale bars are 100 µm except of high magnification in C that is 50 µm. (<b>D</b>) Box-and-whisker plots and (<b>E</b>) standard deviation of ECM angle of orientation. Error bars represent 5–95% confidence intervals. n = 2 with quadruplicates.