ECFCs attached and aligned on fibrin hydrogel microfibers.

<p>(<b>A</b>) Schematic of experimental procedure including electrospinning, ECFC seeding, and tumbling. Drawing not to scale. SEM of critical-point dried fibrin microfiber showing aligned topography on the microfiber surface. Scale bar is 10 µm. (<b>B–D</b>) Confocal z-stack image reconstructions of ECFCs seeded on fibrin hydrogel microfibers horizontally aligned after 5 days in culture. F-actin (phalloidin staining) is shown in green, EC-specific markers (VECad, CD31, or vWF) in red, and nuclei in blue. Yellow arrows indicate the direction of stretching and nanotopography on microfiber surface. Scale bars are 50 µm. n≥2 per stain with quadruplicates.