Shown are radial orientation functions (top row, compare Fig 3) and intrinsic radial orientation obtained by aligning autocorrelograms before average (bottom row, compare Fig 5) of cells treated with 10 μM nocodazole (left column) or DMSO alone (right column). Population averages are shown, offset (value at 0°) subtracted. Direction of strain was 0°. Bars are standard error of mean; for clarity only highest and lowest value at each condition shown. Sample sizes were for nocodazole treatment 58, 98, 87, 52, and 111; for DMSO alone 83, 88, 76, 95, and 107, both in order from long to short duration.</p
(A) Exogenously supplied 100 mM H2O2 suppressed microtubule orientation into well-ordered circumfere...
<p>α = 0 means the ideal functional orientation centrosome-down, and α = 180° means the opposite, no...
<p>(A) Similar numbers of MSCs adhered to all PMMA surfaces after four hours as quantified by DAPI s...
Shown are exemplary micrographs of cells treated with 10 μM nocodazole (A, B) and control cells trea...
Autocorrelograms of individual cells were aligned before calculating the radial orientation function...
A. Control of microtubules depolymerization upon nocodazole treatment. Maximum intensity projections...
<p>Cyclic stretch (20%, 2 h) was applied to the cells after pretreatment with either 1 µM nocodazole...
<p>Indirect immunostaining of tubulin in Jurkat cells attached to the stimulatory substrate. Represe...
<p>Confocal z-stack image reconstructions of ECFCs seeded on fibrin microfibers for 24 hrs followed ...
<p>Each compound was added into culture medium at the following final concentration: nocodazole (0.3...
From left to right: actin, microtubules, and vimentin. Correlograms were aligned and normalized by t...
Additional file 1: Figure S1. (A) A phase contrast image of TCPS surface. Bar, 100 μm. (B) An images...
<p>(A–C). Microtubules visualized by tubulin-GFP fusion. For A and B, two focal planes are shown, to...
<p>Cells were treated for 10 min with A) 0.32 µM or B) 10 µM nocodazole, then immediately fixed in P...
<p>(A) Time projections (the maximum intensity projections of all time points) of time-lapse sequenc...
(A) Exogenously supplied 100 mM H2O2 suppressed microtubule orientation into well-ordered circumfere...
<p>α = 0 means the ideal functional orientation centrosome-down, and α = 180° means the opposite, no...
<p>(A) Similar numbers of MSCs adhered to all PMMA surfaces after four hours as quantified by DAPI s...
Shown are exemplary micrographs of cells treated with 10 μM nocodazole (A, B) and control cells trea...
Autocorrelograms of individual cells were aligned before calculating the radial orientation function...
A. Control of microtubules depolymerization upon nocodazole treatment. Maximum intensity projections...
<p>Cyclic stretch (20%, 2 h) was applied to the cells after pretreatment with either 1 µM nocodazole...
<p>Indirect immunostaining of tubulin in Jurkat cells attached to the stimulatory substrate. Represe...
<p>Confocal z-stack image reconstructions of ECFCs seeded on fibrin microfibers for 24 hrs followed ...
<p>Each compound was added into culture medium at the following final concentration: nocodazole (0.3...
From left to right: actin, microtubules, and vimentin. Correlograms were aligned and normalized by t...
Additional file 1: Figure S1. (A) A phase contrast image of TCPS surface. Bar, 100 μm. (B) An images...
<p>(A–C). Microtubules visualized by tubulin-GFP fusion. For A and B, two focal planes are shown, to...
<p>Cells were treated for 10 min with A) 0.32 µM or B) 10 µM nocodazole, then immediately fixed in P...
<p>(A) Time projections (the maximum intensity projections of all time points) of time-lapse sequenc...
(A) Exogenously supplied 100 mM H2O2 suppressed microtubule orientation into well-ordered circumfere...
<p>α = 0 means the ideal functional orientation centrosome-down, and α = 180° means the opposite, no...
<p>(A) Similar numbers of MSCs adhered to all PMMA surfaces after four hours as quantified by DAPI s...