miR-150 promotes the cell growth and inhibits the cell apoptosis of breast cancer cells.

<p>(A–B) Proliferation curves of breast cancer cells transfected with miR-150 mimics (150- mim), miR-150 inhibitors (150-inh) and matched negative control (NC) (150- mim NC or 150-inh NC). Cell survival was determined by MTT assay. * p<0.01, One-way ANOVA compared to matched NC, Mock or PBS. (C) Anchorage-independent growth as determined by soft agar colony formation assay. (D) Percentage of BrdU⁺ cells determined by flow cytometric analysis of BrdU immunostaining and representative flow cytometric histograms of BrdU immunostaining in MCF-7 or MDA-MB-231 cells transfected with miR-150 mimics, inhibitors and matched negative control. (E) Cell apoptosis of MCF-7 or MDA-MB-231 cells was performed after transfected with miR-150 mimics, inhibitors and matched NC. Data are the mean of three determinations and shown is representative of three experiments that gave similar results.