Reduction in miR-150 suppresses tumor growth in MDA-MB-231 cells xenografts implanted in BALB/c-nu mice.

<p>(A) Tumor volume of mice inoculated with MDA-MB-231 cells that stably expressing miR-150 inhibitor were measured up to 5 weeks. **P<0.01; ***P<0.001, One-way ANOVA compared to untreated, vector or NC groups. (B) Mean ± SD tumor weight of tumor-bearing mice upon necropsy (n = 8/group). **P<0.01, One-way ANOVA. (C) Expression of miR-150 normalized to U6, in the tumors of tumor-bearing mice, was determined by qRT-PCR. ***P<0.001, One-way ANOVA, comparing miR-150 inhibitors to untreated, vector or NC groups. (D) Expression of P2X₇ mRNA relative to GAPDH mRNA, in the tumors of tumor-bearing mice, determined by qRT-PCR. ***P<0.001, One-way ANOVA, comparing miR-150 inhibitor to untreated, vector or NC groups. (E) Immunohistochemical staining (×400) for P2X₇ receptor, PCNA or TUNEL.