<p>BMDCs were stimulated for 24<i>P. brasiliensis</i> (Pb) (MOI 1), ATP, LPS, LPS and Pb (100 ng/ml for 2 h before fungal infection), Pb and ATP (added 1 h before co-culture incubation time completion). As a control, BMDMs were primed with LPS, followed by ATP, as previously described. The supernatants from the cultures were harvested for (A) IL-1β, (C) TNF-α and (D) IL-6 assays using ELISA. (B) Pro-IL-1β was assayed in the cell lysate of BMDCs that were infected or not with <i>P. brasiliensis</i> for 24 h. The data are expressed as the mean ± the SD of two to three independent experiments conducted in triplicates. *denotes <i>p</i>≤0.05 compared with not infected cells.</p
<p>Groups of WT and 5-LO<sup>−/−</sup> mice were infected with 10<sup>6</sup> yeasts. (A) Survival r...
<p>A. <i>P. brasiliensis</i> colony-forming units (CFU) recovered from pulmonary tissues (n = 8 mice...
<p>(<b>A, B</b>) IFN-γ-primed and unprimed macrophages were treated with 1MT or left untreated and t...
<p>BMDMs were stimulated for 24<i>P. brasiliensis</i> (Pb) (MOI 1), ATP, LPS, LPS and Pb (100 ng/ml ...
<p>(A) BMDMs were obtained from WT, IL-1R1<sup>−/−</sup> or NLRP3<sup>−/−</sup> C57BL/6 mice and wer...
<p>(A, B) BMDCs were pretreated for 1 h with the indicated concentrations (<a href="http://www.plosn...
<p>BMDCs were pretreated for 1(<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pnt...
<p>Cytokine levels for TNF-α (A), IFN-γ (B), IL-1β (C), IL-6 (D), CXCL-1/KC (E) and CXCL-2/MIP-2 (F)...
<p>(A, B) BMDMs and (C, D) BMDCs were pretreated for 1 h with the indicated concentrations (<a href=...
<p>(Left) Macrophages were harvested from the peritoneal cavity of WT and 5-LO<sup>−/−</sup> mice an...
Protective immunity in paracoccidioidomycosis is mainly mediated by cellular immunity. the role of B...
Monocytes and macrophages play a central role in innate and adaptive immune response against systemi...
<p>Lungs were removed, leukocytes obtained and the number of pDCs evaluated. The pDCs were character...
Paracoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe...
Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (PCM), the most preva...
<p>Groups of WT and 5-LO<sup>−/−</sup> mice were infected with 10<sup>6</sup> yeasts. (A) Survival r...
<p>A. <i>P. brasiliensis</i> colony-forming units (CFU) recovered from pulmonary tissues (n = 8 mice...
<p>(<b>A, B</b>) IFN-γ-primed and unprimed macrophages were treated with 1MT or left untreated and t...
<p>BMDMs were stimulated for 24<i>P. brasiliensis</i> (Pb) (MOI 1), ATP, LPS, LPS and Pb (100 ng/ml ...
<p>(A) BMDMs were obtained from WT, IL-1R1<sup>−/−</sup> or NLRP3<sup>−/−</sup> C57BL/6 mice and wer...
<p>(A, B) BMDCs were pretreated for 1 h with the indicated concentrations (<a href="http://www.plosn...
<p>BMDCs were pretreated for 1(<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pnt...
<p>Cytokine levels for TNF-α (A), IFN-γ (B), IL-1β (C), IL-6 (D), CXCL-1/KC (E) and CXCL-2/MIP-2 (F)...
<p>(A, B) BMDMs and (C, D) BMDCs were pretreated for 1 h with the indicated concentrations (<a href=...
<p>(Left) Macrophages were harvested from the peritoneal cavity of WT and 5-LO<sup>−/−</sup> mice an...
Protective immunity in paracoccidioidomycosis is mainly mediated by cellular immunity. the role of B...
Monocytes and macrophages play a central role in innate and adaptive immune response against systemi...
<p>Lungs were removed, leukocytes obtained and the number of pDCs evaluated. The pDCs were character...
Paracoccidioidomycosis (PCM) is a systemic fungal infection, endemic in Brazil, that leads to severe...
Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis (PCM), the most preva...
<p>Groups of WT and 5-LO<sup>−/−</sup> mice were infected with 10<sup>6</sup> yeasts. (A) Survival r...
<p>A. <i>P. brasiliensis</i> colony-forming units (CFU) recovered from pulmonary tissues (n = 8 mice...
<p>(<b>A, B</b>) IFN-γ-primed and unprimed macrophages were treated with 1MT or left untreated and t...