<i>P. brasiliensis</i> does not activate the inflammasome but induces the production of pro-IL-1β in BMDMs.

<p>BMDMs were stimulated for 24<i>P. brasiliensis</i> (Pb) (MOI 1), ATP, LPS, LPS and Pb (100 ng/ml for 2 h before fungal infection), Pb and ATP (the latter added 1 h before co-culture incubation time completion). As a control, BMDMs were primed with LPS, followed by ATP, as previously described. The supernatants from the cultures were harvested for (A) IL-1β, (C) TNF-α and (D) IL-6 assays using ELISA. (B) Pro-IL-1β was assayed in the cell lysate of BMDCs that were infected or not with <i>P. brasiliensis</i> for 24 h. The data are expressed as the mean ± the SD of two to three independent experiments conducted in triplicates. *denotes <i>p</i>≤0.05 compared with not infected cells.