Proliferation of CTS-treated cells.

<p>A) Normal MCF10A cells exhibited a concentration-dependent decrease in BrdU incorporation (proliferation) when treated with 500 nM ouabain, 50 nM bufalin or 100 nM bufalin for 2 hours (p<0.01). MCF7, MCF10CA1, and MDA-MB-231 cells showed no significant decrease (p>0.05) in proliferation under comparable conditions. MDA-MB-231 cells treated with 50 nM or 100 nM bufalin had p-values of 0.057 and 0.061, respectively. B) High ouabain treatment (500 nM) for 24 hours caused a decrease in proliferation of MDA-MB-231 and MCF10CA1 cells, but no change to proliferation of MCF7 cells. Low ouabain or bufalin treatment had no effect on proliferation of any cancer cells tested.