Apoptosis in normal and cancerous cells.

<p>Ouabain causes MCF10A cells to undergo apoptosis, but not MCF7, MDA-MB-231, or MCF10CA1 cells. DNA fragmentation (free 3’-OH ends) was visible by FITC (green) staining using the TUNEL assay when MCF10A cells were treated with 500 nM ouabain or 50 nM bufalin for 6 hours. DNaseI was included as a positive control for DNA fragmentation of in MCF10A and MCF10CA1 cells. DAPI (blue) was used to counter-stain the nucleus, and images were collected using Zeiss Confocal Microscope and Imaging Software.