Use of IL-10-blocking antibodies to assess IL-10 effects on MØs and DC cytokines.

<p>Experiments were performed as in Figure 2, except MØs and DC from B6 mice were preincubated with 3μg/ml of either an IL-10-blocking (αIL-10) or isotype control antibody for 30 min before adding Bb (MOI=10) at 37°C. Each symbol represents the average of triplicate samples from at least three separate experiments. Statistically significant (P<0.05) values are indicated between Bb-stimulated versus unstimulated APCs (*), or stimulated isotype Ab-treated B6 versus αIL-10-treated APCs (**).