DC-derived IL-10 is required for modulation of EAE by MOG-CD8+ T cells.

<p>(<b>A</b>) Effect of DC-derived IL-10 on CD4+ T cell proliferation was evaluated using a thymidine-incorporation assay. Magnetically sorted DC from OVA-CD8+ or MOG-CD8+ recipient mice were co-cultured with CD4+ T cells from MOG_35–55/CFA immunized mice. 4 µg/ml of anti-IL-10 antibody or IgG isotype control was added to the indicated cultures. (<b>B</b>) WT MOG-CD8+ and OVA-CD8+ T cells were transferred to either naïve wild-type (left panel) or IL-10−/− (right panel) mice, followed by EAE induction. Mean EAE scores are plotted on the y-axis vs. days post-transfer on the x-axis. Data represent two independent experiments, with 6–8 mice per group (*p<0.05).