<p>MØs (A) and DCs (B) expanded from B6 and IL-10<sup>-/-</sup> mice were cultured with Bb for 24h before staining with fluorescence antibodies specific for the indicated surface molecules and analyzed by flow cytometry. Data are reported both as percentage of positive cells (left panels) and mean fluorescent intensity (MFI). Each bar represents triplicate samples from at least three separate experiments. Statistically significant (P<0.05) values are indicated compared to unstimulated (*) or Bb-stimulated APCs (**). </p
<p>(<b>A–C</b>) Phenotypic changes during <i>in vitro</i> proliferation. CD4<sup>+</sup>CD25<sup>+</...
<p>Splenic B cells were purified from naïve or <i>B. microti</i>-infected mice by MACS as described ...
<p>DCs were cultured in the presence of sTM from day 4. Neutralizing anti-mouse IL-10 mAb (clone: 2A...
<p>MØs (upper panel) or DCs (lower panel) derived from either wild type (wt) C57BL/6 or IL-10<sup>-/...
<p>Experiments were performed as in Figure 2, except MØs and DC from B6 mice were preincubated with ...
<p>A. MØs (left panels) and DCs (right panels) were cultured in 96-well plates for 15 h before loadi...
<p>(A–J) BMDM were stimulated and cultured as indicated in <a href="http://www.plosone.org/article/i...
<p>(A) Mouse BMDCs were cultured for 24 h in the presence of LPS, MPL, or dLOS, and expression of CD...
<p>CD19<sup>+</sup> B cells were isolated by MACS from naïve mice and cultured for five days with CD...
<p>(A–F) Primary B cells from either wild type C57BL6 (B6) mice or IL10−/− mice (as indicated) were ...
<p>Freshly isolated PBMCs (n = 12) were incubated in absence or in presence of BPA at the indicated ...
<p>Macrophages from wild type (open bar) or p110γ<sup>/−</sup> mice (solid bar) were incubated in 24...
<p>PBMC from healthy individuals were stimulated with HBsAg, with or without IL-10. After overnight ...
<p>a: Representative flow-cytometry histograms showing IL-10-producing cells identified within CD1d<...
With the recently increased understanding of the roles of B cells in immune response and autoimmune ...
<p>(<b>A–C</b>) Phenotypic changes during <i>in vitro</i> proliferation. CD4<sup>+</sup>CD25<sup>+</...
<p>Splenic B cells were purified from naïve or <i>B. microti</i>-infected mice by MACS as described ...
<p>DCs were cultured in the presence of sTM from day 4. Neutralizing anti-mouse IL-10 mAb (clone: 2A...
<p>MØs (upper panel) or DCs (lower panel) derived from either wild type (wt) C57BL/6 or IL-10<sup>-/...
<p>Experiments were performed as in Figure 2, except MØs and DC from B6 mice were preincubated with ...
<p>A. MØs (left panels) and DCs (right panels) were cultured in 96-well plates for 15 h before loadi...
<p>(A–J) BMDM were stimulated and cultured as indicated in <a href="http://www.plosone.org/article/i...
<p>(A) Mouse BMDCs were cultured for 24 h in the presence of LPS, MPL, or dLOS, and expression of CD...
<p>CD19<sup>+</sup> B cells were isolated by MACS from naïve mice and cultured for five days with CD...
<p>(A–F) Primary B cells from either wild type C57BL6 (B6) mice or IL10−/− mice (as indicated) were ...
<p>Freshly isolated PBMCs (n = 12) were incubated in absence or in presence of BPA at the indicated ...
<p>Macrophages from wild type (open bar) or p110γ<sup>/−</sup> mice (solid bar) were incubated in 24...
<p>PBMC from healthy individuals were stimulated with HBsAg, with or without IL-10. After overnight ...
<p>a: Representative flow-cytometry histograms showing IL-10-producing cells identified within CD1d<...
With the recently increased understanding of the roles of B cells in immune response and autoimmune ...
<p>(<b>A–C</b>) Phenotypic changes during <i>in vitro</i> proliferation. CD4<sup>+</sup>CD25<sup>+</...
<p>Splenic B cells were purified from naïve or <i>B. microti</i>-infected mice by MACS as described ...
<p>DCs were cultured in the presence of sTM from day 4. Neutralizing anti-mouse IL-10 mAb (clone: 2A...