Effects of IL-10 on Bb-induced ROS and RNI production by APCs.

<p>A. MØs (left panels) and DCs (right panels) were cultured in 96-well plates for 15 h before loading with DCF for 15 min. Cells were then pre-treated with or without rIL-10 (2ng/10⁵ cells) or the NADPH oxidase inhibitor DPI (10μM) for an additional 30 min prior to Bb-stimulation; 0.01% H₂O₂ provided a positive control. ROS production was assessed by detecting the relative fluorescence intensity (RFU) values for up to 40 min post-stimulation. Top: Data are expressed as raw RFU values per min over a 40 min interval (Left: MØs, Right: DCs). Middle: Slope of RFU for each condition is calculated as changes in RFU over time for the first 30 min. Data are representative of three separate experiments. Statistically significant (P<0.05) values are indicated compared to unstimulated control (*) or Bb stimulation (**). B. MØs (left panels) and DCs (right panels) were stimulated with Bb for 24h. Supernatant nitric oxide (nitrite) levels were assessed using the Griess assay. Statistically significant (P<0.05) values are indicated compared to unstimulated controls (*) or Bb stimulation (**).