FJU-C4 compound inhibited pro-inflammatory cytokine secretion and prevented LPS-induced mouse death.

<p>Mice were pretreated with different doses of FJU-C4 compound as indicated and then stimulated by LPS (15 mg/kg) in PBS buffer (A) The serum of the mice (n = 3 for each group) was collected and measured for TNFα and IL-1β at 6 and 24 hours, respectively, by using ELISA kit with a standard protein curve. (B) LPS-treated mice with/without FJU-C4 compound treatment (as indicated) were observed for 72 hours and their survival rates were recorded. Mice in the control group were administered LPS alone with solvent (1% DMAO in PBS) (n = 15); one group was administered LPS plus 5 mg/kg FJU-C4 (n = 15), and the other group was administered LPS plus 10 mg/kg FJU-C4 (n = 20). The animal model data were pooled for three independent experiments. *<i>p</i>< 0.05 versus the LPS group.