Chtx protects mice from endotoxic shock.

<p>Chtx and FAg inhibit induction of pro inflammatory cytokines in vivo by LPS. Eight to ten weeks old BALB/c mice were injected IP with 15 mg/Kg body wt of LPS with or without FAg (100 µg) and chtx 250 µg and blood was collected by heart puncture in heparinised tubes after 2 hrs. Plasma levels of TNF-α, IL-1 β and IL- 6 (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002717#ppat-1002717-g005" target="_blank">Fig. 5 <b>A</b></a>) and IL-10 and nitrite (<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002717#ppat-1002717-g005" target="_blank">Fig. 5 <b>B</b></a>) were quantified as described earlier. Mean ± SEM of 10 independent experiments are shown. * P<0.001, versus LPS treated cells (Student's t-test). (<b>C</b>) C57 BL/6 mice were injected IP with lethal dose of LPS (60 mg/Kg body wt) with or without chtx (250 ug) and mortality was scored. (N = 15). (<b>D</b>) C57 BL/6 mice were injected IP with lethal dose of LPS (60 mg/Kg body wt) and chtx was injected later as shown and mortality scored. N = 17 for LPS, N = 10 for LPS+Chtx 6 hrs, N = 16 for LPS+Chtx 24 hrs and LPS+chtx 48 hrs. (<b>E</b>) BALB/c mice were injected IP with lethal dose of LPS (15 mg/Kg body wt.) with or without FAg (100 µg) or AgW (50 µg) or Chtx (250 µg) and the mortality was scored. N = 10.