MXI1 cDNA can rescue the effect of miR-155.

<p>(A) U87 cells were transfected with empty vector or with MXI1 cDNA, and an EdU incorporation assay was analyzed after 48 h (scale bar, 50 µm). (B) U87 cells were transfected with empty vector or with MXI1 cDNA, and the growth of the cells was investigated using an MTT assay after 72 h. (C) U87 cells were co-transfected with either mimic control and empty vector or miR-155 mimic and MXI1-cDNA. An EdU incorporation assay was analyzed after 48 h (scale bar, 50 µm). The EdU incorporation rate was expressed as the ratio of the number of EdU-positive cells to the total number of Hoechst 33342-positive cells. The average values ± SD of three separate experiments were plotted, ** <i>P</i><0.01. (D) U87 cells were co-transfected with either mimic control and empty vector or miR-155 mimic and MXI1-cDNA, and the cell growth was investigated using an MTT assay after 72 h. The absorbance at 490 nm was normalized to the appropriate control to determine cell viability. Values represent the means ± SD.