miR-152, -10b-5p and miR-34c-3p modulated proliferation and apoptosis of MM cells.

<p>Since the three miRNAs have different methylation frequencies and expression levels in MM cell lines, H929 cells were transfected with miR-152, -10b-5p and -34c-3p mirVana miRNA respectively; IM9 cells were transfected with miR-152 and -34c-3p mirVana miRNA respectively; MM1S cells were used to overexpress miR-10b-5p. All of the cells transfected with negative control mimics used as control cells. (A) The indicated transfected cells were cultured in presence or absence with primary BMSCs for 72 hours, following by [H]³ thymidine uptake assay. NC-transfected cells in absence with BMSCs were defined as 1.0 and regarded as the control group. ^*<i>P</i><0.05. (B) MM cells (NC-, miR-152-, -10b-5p-, or miR-34c-3p-transfected) were harvested at 48 or 72 hours after transfection. Whole cell lysates were subjected to western blotting using anti-PARP, -Caspase 9, -Caspase 3 and -α-tubulin antibodies.