K_Ca3.1 inhibition TGFβ1 and bFGF-induced myofibroblast contraction.

<p><b>a</b>) Myofibroblast collagen gel contraction was increased following TGFβ1 stimulation and this was inhibited by TRAM-34 200 nM in both IPF (n=3) and NFC (n=3) donors (data shown is pooled IPF and NFC which did not differ, n=6)(<i>P</i>=0.0014, repeated measures ANOVA, <i>P</i>=0.0023 for TGFβ1 compared to control, <i>P</i>=0.0026 for TRAM-34 compared to TGFβ1 corrected by Bonferroni’s multiple comparisons test). <b>b</b>) TGFβ1-dependent myofibroblast collagen gel contraction was also inhibited by ICA-17043 100 nM (n=6) (All groups; repeated measures ANOVA, <i>P</i>=0.0002, TGFβ1 versus control, <i>P</i>=0.0002 and for ICA-17043 versus TGFβ1, <i>P</i>=0.0006, corrected by Bonferroni’s multiple comparisons test). <b>c</b>) Similarly, myofibroblast collagen gel contraction was increased following bFGF stimulation and was also inhibited with 24h pre-treatment with TRAM-34 200 nM (All groups; repeated measures ANOVA <i>P</i><0.0001, for bFGF compared to control, <i>P</i><0.0001, and for TRAM-34 compared to bFGF, <i>P</i><0.0001 (corrected by Bonferroni’s multiple comparisons test). <b>d</b>) Similarly, 24h pre-treatment with ICA-17043 100 nM significantly reduced bFGF-dependent myofibroblast collagen gel contraction (<i>P</i>=0.0007, repeated measures ANOVA) (<i>P</i>=0.0005 for bFGF versus control and <i>P</i>=0.0053 for ICA-17043 versus bFGF, corrected by Bonferroni’s multiple comparisons test). Data represented as mean±SEM for all the above figures.