Myofibroblast proliferation is inhibited by K_Ca3.1 channel block.

<div><p>Constitutive unstimulated wound healing is not altered by K_Ca3.1 blockade, and growth factor-stimulated healing is not inhibited by TRAM-7 or TRAM-85 .</p> <p><b>a</b>) Myofibroblast proliferation was increased following 48h of stimulation with FBS and significantly reduced by TRAM-34 (200 nM). <b>b</b>) An example of the wound created in a confluent monolayer of myofibroblasts in the wound healing assay and how it heals over the 48 hours. <b>c</b>) This graph displays that over the time course of the wound healing assay no significant differences were found between NFC and IPF donors in response bFGF, similar results were seen with FBS but results are not shown. <b>d</b>) TRAM-34 does not inhibit wound healing in the absence of mitogenic stimulation. <b>e</b>) FBS significantly increases wound healing (*<i>P</i>=0.0168, Paired t-test) but the molecules TRAM-7 and TRAM-85 which are structurally related to TRAM-34 do not have K_Ca3.1 channel-blocking activity and do not inhibit mitogen-dependent wound healing. Data represent mean±SEM for all figures a, c, d and e.</p>