LBP protects retinal amacrine cells against ischemia-reperfusion-induced damage.

<p>Retinal amacrine cells were stained with anti-ChAT antibody. <b>A, B</b>: Representative micrographs of retinal sections stained with anti-ChAT antibody at 24 h (<b>A</b>) or 7 days (<b>B</b>) after ischemia. <b>C:</b> Quantitative analysis of ChAT-positive cells in the GCL and INL (mean ± SEM, n = 5). <b>D, E:</b> Representative immunoblot of ChAT levels in whole retina (upper panel) at 24 h (<b>D</b>) or 7 days (<b>E</b>) after ischemia and densitometric analysis of ChAT expression relative to loading control (lower panel) (mean ± SEM, n = 5). Control: sham-operated animal, I/R: vehicle-treated animal with 1 h ischemia, and LBP+I/R: LBP-pretreated animal with 1 h ischemia. *<i>p</i><0.05, ***<i>p</i><0.001 compared to control, # <i>p</i><0.05, ### <i>p</i><0.001 compared to I/R at the same time point. Scale bar: 20 µm. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer.