Increased immunostaining of bFGF is a hallmark of ischemia-reperfusion in the rat retina.

<p>The images display representative slices of a control retina (<i>upper panel</i>) and a retina obtained 3 d after reperfusion (<i>lower panel</i>). Specimens were labeled with antibodies against bFGF (<i>red</i>) and vimentin (<i>green</i>). Co-labeling yielded a <i>yellow</i> merge signal, and cell nuclei were labeled with Hoechst 33258 (<i>blue</i>, scale bar, 20 µm). Note increasing vimentin and bFGF labeling in the outer nuclear layer (ONL) after ischemia as compared to the control tissue. <i>Unfilled arrowhead</i>, ganglion cell soma; <i>arrow</i>, Müller cell soma; <i>filled arrowhead</i>, Müller cell process. GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; OPL, outer plexiform layer; PRS, photoreceptor segments.