Abolishment of the protective effects of LBP after ischemia-reperfusion by the HO-1 inhibitor in retina.

<p>The involvement of HO-1 in the protective effects of LBP after ischemia-reperfusion in the retina was determined using the specific HO-1 inhibitor, ZnPP. <b>A</b>: Representative micrographs of retina sections stained with the RGC specific marker, Rbpms at 24 h after ischemia <b>B</b>: Quantitative analysis of Rbpms-positive cells in the retinal ganglion cell layer (mean ± SEM, n = 5). <b>C</b>: Representative micrographs of retina sections stained with the amacrine specific marker anti-ChAT at 24 h after ischemia. D: Quantitative analysis of ChAT-positive cells in the GCL and INL (mean ± SEM, n = 5). I/R: vehicle-treated animal with 1 h ischemia, LBP+I/R: LBP-pretreated animal with 1 h ischemia, LBP+I/R+ZnPP: LBP-pretreated animal with ZnPP injection 24 h before 1 h ischemia. **<i>p</i><0.01, ***<i>p</i><0.001 compared to I/R, ## <i>p</i><0.01, ### <i>p</i><0.001 compared to LBP+I/R. Scale bar: 20 µm. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer.