LBP upregulates the expression of HO-1 in the retina after ischemia-reperfusion.

<p>HO-1 expression in the retina was determined using immunofluorescent staining and immunoblotting. <b>A, B:</b> Representative micrographs of retinal sections stained with anti-HO-1 antibody at 24 h (A) or 7 days after ischemia. <b>C, D:</b> Representative immunoblot of HO-1 protein levels in whole retina at 24 h (<b>C</b>) and 7 days (<b>D</b>) after ischemia (upper panel) and densitometric analysis of HO-1 expression relative to the loading control (lower panel, mean ± SEM, n = 5). Control: sham-operated animal, I/R: vehicle-treated animal with 1 h ischemia, LBP+I/R: LBP-pretreated animal with 1 h ischemia, SF+I/R: sulforaphane-pretreated animal with 1 h ischemia. **<i>p</i><0.01, ***<i>p</i><0.001 compared to control, # <i>p</i><0.05 compared to I/R. Scale bar: 20 µm. GCL: ganglion cell layer; INL: inner nuclear layer; ONL: outer nuclear layer.