Combination treatment of 1D11 and CY reduces the number of splenic MDSCs and promotes their re-differentiation.

<p>Four weeks after 4T1 tumor inoculation, cell suspensions were prepared from spleen and MDSCs were analyzed by FACS, gating on live CD45⁺ cells. (A-B) Proportion and number of Gr1⁺CD11b⁺ cells in the spleens. Typical FACS plots are shown in (A, gating on total live splenic CD45⁺ cells) and summary of data pooled from three experiments are shown in (B, percent of PBS control group, Means±SEM, N = 10∼13). (C) Absolute number of Gr1⁺CD11b⁺ cells in the spleen (N = 11, pooled from two separate experiments). (D-E) Expression of I-A/I-E and CD80 on Gr1⁺CD11b⁺ splenic cells. (D) Typical FACS plots (gating on Gr1⁺CD11b⁺ cells) and (D) summary of data pooled from three separate experiments (Means±SEM, N = 9). Comparison of indicated groups, *p<0.05, ** p<0.01, ***p<0.001.