Combination treatment of 1D11 and CY promotes tumor infiltration of IFNγ-producing T cells.

<p>Four weeks after 4T1 tumor inoculation, cell suspension was prepared from tumor tissues. (A-B) Proportion of TCRβ⁺ T cells in total tumor infiltrating CD45⁺ leukocytes. Typical flow plots are shown in (A), and summary of data from three separate experiments is shown in (B, Means±SEM, N = 14∼20). (C-F) IFNγ expression by CD8 and CD4 TILs. Intracellular expression of IFNγ was analyzed by FACS, gating on live CD45⁺TCRβ⁺CD8⁺ cells (C-D) or CD45⁺TCRβ⁺CD4⁺ cells (E-F). Data shown are typical FACS plots (C, E) and summary of data (D, F) from three separate experiments (Means±SEM, N = 9). Comparison of indicated groups, * p<0.05, ** p<0.01. <b>(</b>G, H<b>)</b> Normal WT Balb/c mice and IFNγ KO mice were inoculated with 4T1 cells and treated with 1D11+CY in the same manner. (G) Incidence of 4T1 tumor in mice treated with PBS. (H) Incidence of 4T1 tumor in mice treated with 1D11 and CY. Data are shown as percent tumor free mice (%, KO mice N = 3, WT mice N = 5), which are representatives of two separate experiments with similar results.