FACS analysis of stromal cell populations in spleen from p110δ^WT/WT and p110δ^D910A/D910A mice.

<p>Spleens from p110δ^WT/WT and p110δ^D910A/D910A mice were processed and stained with anti-CD45, -TER119, -CD31, and -gp38 mAb. A) Representative gating strategy for the analysis of stromal cell populations. Stromal cells were gated via the exclusion of dead, CD45-, and TER119-positive cells. B) Quantification of the percentage and absolute number of stromal cell populations in spleens of p110δ^WT/WT and p110δ^D910A/D910A mice (<i>n</i> = 3 experiments/spleen, 6 mice/group). Student's <i>t</i>-test, *p<0.05.