Increased DC population after M-HIFU treatment.

<p>Three mice from each group were sacrificed six days after surgery or HIFU plus surgery treatment. Single cell suspension was prepared from the pooled spleens or TDLNs; and CD11c⁺ cell population was determined by flow cytometry. Representative results from three independent experiments are shown. <i>A</i>, <i>C</i>, Representative flow patterns in detecting CD11c⁺ cells in spleen (<i>A</i>) and TDLNs (<i>C</i>). <i>B</i>, <i>D</i>, Statistical significance in the increase of the DC population in spleen (<i>B</i>) and TDLNs (<i>D</i>) based on the results of <i>A</i> and <i>C,</i> respectively. <i>E</i>, <i>F</i>, Evaluation of DC stimulation by detecting CD80-positive (<i>E</i>) and CD86-positive (<i>F</i>) CD11c⁺ cells in spleen and TDLNs. *p<0.05, ***p<0.0001.