Effects of glutathione and 2,2′-dipyridyl on the growth profiles of cells in the presence of sublethal concentrations of kanamycin.

<p>Growth profiles of MG1655 WT and the KO mutants <i>ΔravA</i>, <i>ΔviaA</i> and <i>ΔravAviaA</i> grown aerobically in LB at 37°C without (A, and D) or with kanamycin (B, and E) or streptomycin (C and F). Selected cultures were further supplemented with 750 µM of GSH (A–C) or 250 µM DP (D–F). Kanamycin was added at 4 µg/mL final concentration. Error bars were derived from three independent cultures for each strain and for each condition. In some instances, the error bars are smaller than the symbols used and cannot be seen. (G) DHR fluorescence measurements normalized by OD₆₀₀ for MG1655 WT+p11, <i>ΔravAviaA</i>+p11, <i>ΔravAviaA</i>+pRV and <i>ΔravAviaA</i>+pR_K52QV grown aerobically to late log phase in LB at 37°C supplemented with 4 µg/mL kanamycin in the presence or absence of 8 mM GSH or 250 µM DP. Error bars were derived from three independent cultures for each strain and for each condition. To highlight the statistical significance, the p-values for <i>ΔravAviaA</i>+p11 vs. WT+p11 (indicated with *) and <i>ΔravAviaA</i>+pRV vs. WT+p11 (indicated with **), in the presence of kanamycin, are given in the upper-right corner of the panel.