Growth profiles of cells in the presence of sublethal concentrations of aminoglycosides.

<p>Growth profiles for MG1655 WT and the KO mutants <i>ΔravA</i>, <i>ΔviaA</i> and <i>ΔravAviaA</i> grown aerobically in LB at 37°C over 24 hours. Growth of cells was monitored using OD₆₀₀ readings at the designated time points. The cultures were supplemented as follows: (A) no antibiotics; (B) 4 µg/mL kanamycin; (C) 6 µg/mL streptomycin; (D) 0.5 µg/mL tetracycline; and (E) 1.2 µg/mL chloramphenicol. To confirm the phenotypes observed, <i>ΔravA</i> (F), <i>ΔravAviaA</i> (G) and WT cells (H) were complemented with the plasmids p11 (empty vector control), pR, pRV, pR_K52Q, or pR_K52QV. All cultures in the complementation experiments were supplemented with 4 µg/mL kanamycin for stress induction, and 100 µg/mL ampicillin for plasmid maintenance. Error bars were derived from three independent cultures for each strain and for each condition. Details on the <i>E. coli</i> strains and plasmids used are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085529#pone-0085529-t001" target="_blank">Table 1</a>.