Phenotype of GlmS⁻ mutant <i>E. coli</i>.

<p>(A) Growth of GlmS⁻ mutant <i>E. coli</i> under various media conditions. GlmS⁻ mutant <i>E. coli</i> (CKS1001, open circles) and GlmS⁻ mutant <i>E. coli</i> carrying <i>^E.cGlmS⁺p</i> (closed circles) grown overnight in LB supplemented with 0.2% GlcNAc were diluted 50-fold in minimal media or media supplemented with 0.2% GlcNAc (open triangles) and grown for 24 hrs. Wild-type parental <i>E. coli</i> (CH1436, closed triangles) were grown in the same way in minimal media. Samples were taken at the indicated times for CFU determination on supplemented LB plates. (B) GlmS⁻ mutant (CKS1001) or parental wild-type <i>E. coli</i> (CH1436) carrying ϕ<i>hdeAB</i>p:<i>lacZYA</i> grown overnight in LB supplemented with 0.2% GlcNAc were diluted 50-fold in minimal media and grown for 5 hrs. β-galactosidase activity (A₄₂₀/min/ml) in the supernatants and lysed pellets was determined. (C) GlmS⁻ mutant bacteria (CKS1001) carrying <i>^E.cGlmS⁺pGFP</i> or <i>pGFP</i> grown overnight in LB supplemented with 0.2% GlcNAc were diluted 50-fold in minimal media and cultured for the indicated times. Wild-type parental <i>E. coli</i> (CH1436) carrying <i>pGFP</i> was grown the same way in minimal media. Samples were taken at the indicated times, the supernatant (<i>s</i>) and pellet (<i>p</i>) fractions were isolated, and the fractions were separated by 12% SDS-PAGE for determination of GFP by Western blotting.