Comparison of the effects of Ku-0063794 and rapamycin on protein synthesis in 4E-BP wild-type and 4E-BP-deficient cells under normal and hypertonic conditions.

<p>(A) Wild-type cells and (B) DKO cells were pre-incubated in complete medium in the absence or presence of additional 0.1 M NaCl. After 1 h the cells were further incubated with or without Ku-0063794 (1 µM) or rapamycin (100 nM) for 1 h and total protein synthesis was then measured by the incorporation of [³⁵S]methionine (2 µCi/ml) for 1 h as described in Experimental. The data are the means of triplicate determinations and are expressed as counts per min incorporated per µg of protein ± S.E.M. Significances of differences between incubations with and without each mTOR inhibitor were determined by unpaired t tests: * = p<0.002; ** = p<0.0001; N.S. = not significant.