Up-scaling and hyper-density.

<p>(<b>A</b>) Schematic of the effects of up-scaling: the combination of different lower-density source plates into one higher-density target plate. (<b>B</b>) Comparison of variance in plates pinned with dedicated density-pads (1536, 6144) and plates using up-scaling with or without intra-plate source correction. (<b>C</b>) Colony size distributions obtained by the analysis pipeline without (top) and with (bottom) the intra-plate source correction. (<b>D</b>) Comparison of the percentage of single mutants that can be identified with a significant fitness phenotype at a given cost/replicate level (--- indicate N = 6 at 1536 density). (<b>E</b>) Snapshots of 6144- and 24,576 hyper-density colonies at equal scale (scale bar 1 mm). (<b>F</b>) Zoomed image showing jitter effect on colony placement; red grid represents perfect alignment, blue dots denote actual pin position (scale bar 100 µm). (<b>G</b>) Correlation of fitness measurements obtained with ultra-high- and hyper-density plates.