Relative position of the variants in the Colony Size assay and fluctuation of the best cut-off.

<p>(<b>A</b>) Waterfall distribution of colony sizes, according to median values (standard method). Boxplot representation results from 9 (mutants) or 36 (BRCA1 and Vector) colony size values. The red and blue colors of the boxes indicate the pathogenic and neutral mutations, respectively, according to their prior classification. Box central bar, median; box, interquartile range (50% of the distribution); whiskers, extreme values; dotted horizontal line, median of BRCA1; thick horizontal line, experimental best cut-off (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006096#pgen.1006096.s004" target="_blank">S2 Fig</a>). The distribution of the best cut-off fluctuation, obtained after random sampling (bootstrap), of the 9 mutants and 36 BRCA1 values, is visualized by the pink, grey and light blue areas, that delimit 4%, 90% and 4.9% of the distribution, respectively, which altogether represents a total coverage of 98.9%. (<b>B</b>) Waterfall distribution according to p values (MWW method). The p value assigned to each variant is symbolized by a segment. The upside-down representation facilitates the comparison of the mutation arrangement with the one obtained in <b>A</b>. Arrows pinpoint a modification of the mutation rank depending on the method used. Framed mutations indicate identical p values (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006096#pgen.1006096.s033" target="_blank">S4 Table</a>). Segment colors, thick horizontal line and colored areas, as in <b>A</b>.