Ultra-high-density format data quality and cost efficiency.

<p>(<b>A</b>) Percentage of colonies overgrown. (<b>B</b>) Growth curves based on median colony area fold-increase since pinning (dashed lines indicate fold increase at 12 hrs [6144] or 48 hrs [1536], N = 18 for each colony density). (<b>C</b>) Distribution of fitness resolutions for 1536 and 6144 format (N = replicate numbers). (<b>D</b>) Mode fitness resolutions for a given cost/replicate level (dashed lines indicate equal cost/quality levels, N = replicate numbers). (<b>E</b>) Percentage of single mutants that can be resolved (dashed lines indicate equal cost/quality levels, N = replicate numbers).