Colony size and irregularity for various glucose and agar concentrations.

<p><i>(A, B)</i> Images of <i>FLO11 (A)</i> and <i>flo11Δ (B)</i> on YPD plates containing different glucose (0.5%, 1.0%, 2.0%) and agar (1.5%, 3.0%, 6.0%) concentrations around day 20. <i>(C, D)</i> The expansion of colony area <i>(C)</i> and the irregularity <i>(D)</i> of <i>FLO11</i> (red curves) and <i>flo11Δ</i> (green curves) colonies over the 60-day time course. <i>FLO11</i> colonies (red curves) demonstrated higher maximum colony size <i>(C)</i> with higher irregularity <i>(D)</i> at the colony rim than the <i>flo11Δ</i> colonies (green curves) in all conditions tested. The maximum colony size <i>(C)</i> of both <i>FLO11</i> (red curves) and <i>flo11Δ</i> (green curves) colonies increased with glucose and inversely depended on agar concentrations. The irregularity of <i>FLO11 (D)</i> (red curves) inversely depended on both the agar and the glucose concentrations, compared to the minimal irregularity of <i>flo11Δ (D)</i> (green curves) colonies throughout the time course at all conditions tested. Thinner curves represent different replicates while thicker curves represent their average up until all the replicates were present.