Thin layer chromatography (TLC) analyses of biotransformation of Rb<sub>1</sub>, Rd, Gyp XVII, Rg<sub>3</sub>(<i>S</i>) in (A) and Rb<sub>2</sub>, Rb<sub>3</sub>, Rc in (B) by recombinant BglPm.
- Chang-Hao Cui (515104)
- Jin-Kwang Kim (515105)
- Sun-Chang Kim (515106)
- Wan-Taek Im (515107)
Publication date
January 2014
DOIAbstract
<p>The reaction time was 30: CHCl<sub>3</sub>-CH<sub>3</sub>OH-H<sub>2</sub>O (65∶35∶10, lower phase). (S, S1, S2, S3), ginsenoside standards (PPD type ginsenoside mixtures); Standards: 1, 3, 5, 7, 9, 11, 13; Reaction mixture: 2, 4, 6, 8, 10, 12, 14; 1, Rb<sub>1</sub>; 2, reaction mixture of Rb<sub>1</sub>; 3, Rd; 4, reaction mixture of Rd; 5, Gyp XVII; 6, reaction mixture of Gyp XVII; 7, Rg<sub>3</sub>(<i>S</i>); 8, reaction mixture of Rg<sub>3</sub>(<i>S</i>); 9, Rb<sub>2</sub>; 10, reaction mixture of Rb<sub>2</sub>; 11, Rb<sub>3</sub>; 12, reaction mixture of Rb<sub>3</sub>; 13, Rc; 14, reaction mixture of Rc. Abbreviations: C-K, compound K.</p
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