TLC analysis of reaction products from acceptors and Glc1<i>P</i>.

<p>(A) Reaction products from monosaccharides and Glc1<i>P</i>. Reactions were performed with 2 mg/ml of Lin1839r in the presence of 50 mM monosaccharides and 50 mM Glc1<i>P</i> for 1 h. (B) Time course of the reaction products from glucose and Glc1<i>P</i>. Substrates used were 50 mM glucose and 50 mM Glc1<i>P</i>. The enzyme concentration used was 2 mg/ml. (C) Reaction products after reaction for 2 h using 10 mM Sop₂ and 20 mM Glc1<i>P</i> as substrates. (D) Time course of the reaction products from laminaribiose and Glc1<i>P</i>. The substrates used were 10 mM laminaribiose and 10 mM Glc1<i>P</i>. The enzyme concentration used was 0.5 mg/ml. (A, C) Presence and absence of Lin1839r are represented with ‘+’ and ‘−’, respectively. (B, D) M, marker. Numbers under a line represent reaction time.