GCMS analysis of products formed <i>in vitro</i> with clade II <i>Sa</i>CYP76Fs.

<p>GCMS analysis (extracted ion chromatograms) of products formed <i>in vitro</i> with (<b>A</b>) <i>Sa</i>CYP76F38v1; (<b>B</b>) <i>Sa</i>CYP76F38v2; (<b>C</b>) <i>Sa</i>CYP76F37v1; (<b>D</b>) <i>Sa</i>CYP76F37v2. Assays were performed with a sesquiterpene mixture of α-, β- and <i>epi</i>-β-santalene and α-<i>exo</i>-bergamotene (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075053#pone.0075053.s003" target="_blank">Figure S3</a>) as substrate and microsomes prepared from yeast transformed with <i>Sa</i>CPR, individual clade II candidate <i>Sa</i>CYP76F cDNAs, or (<b>E</b>) empty vector as control. Peak numbers match the numbers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075053#pone-0075053-t001" target="_blank">Table 1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075053#pone-0075053-g001" target="_blank">Figure 1</a>.