Biochemical characterization of IrTPS5 and IrTPS3.

<p>GC-MS analysis of extracted enzyme products resulting from <i>Agrobacterium</i>-mediated transient <i>N</i>. <i>benthamiana</i> co-expression assays of the class II diTPSs IrTPS5 (<b>A</b>) and IrTPS3 (<b>B</b>) with the class I diTPSs <i>Grindelia robusta ent</i>-kaurene synthase (GrEKS) or <i>Marrubium vulgare</i> epoxy-labdane/miltiradiene synthase (MvELS). Reaction products are depicted as extracted ion chromatograms (EIC, <i>m</i>/<i>z</i> 257) with corresponding mass spectra (<b>C</b>) for products: a, <i>ent</i>-copalol (i.e. dephosphorylated CPP); b, <i>ent</i>-kaurene; c, (+)-copalol; d, unidentified diterpene; e, miltiradiene.