The effects of the different incubation times and substrate concentrations on the transglycosylation activity of Os1BGlu4.

<p>TLC analysis of products are shown. The standards are marked as M: marker, including <i>p</i>NPGlc (pG), <i>p</i>NP-<i>β</i>-cellobioside (pC2). <i>p</i>NP marks the position of <i>p</i>-nitrophenol. For the reactions, con is a control reaction without rOs1BGlu4, and 0.5–40, stand for reactions including 0.5 mM <i>p</i>NPGlc, 5 mM <i>p</i>NPGlc, 10 mM <i>p</i>NPGlc, 20 mM <i>p</i>NPGlc, and 40 mM <i>p</i>NPGlc, respectively, while 1, 2 and 3 hours are the incubation times. A: TLC plate visualized by the carbohydrate staining method. B: TLC plate visualized by UV light.