Expression of Pgp mRNAs in xL3 larvae of <i>H. contortus</i> after 24 hours stimulation with increasing concentrations of eosinophil granule proteins.

<p>Real-time RT-PCR experiments were performed in triplicate for each sample using two distinct cDNA preparations per sample. mRNA expression levels were normalized using non-stimulated xL3. The mRNA fold changes were calculated using three distinct reference genes (<i>gapdh, actin</i> and <i>β-tubulin</i>). ** Significant difference from control values after Man-Whitney test analyses (<i>P<0.01</i>).